Publications

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2025

  • D.E. Reynolds*, Y. Roh*, U. Chintapula, E. Huynh, P. Vallapureddy, H. H. Tran, D. Lee, M. G. Allen, X. Xu, J. Ko. “Vertically Aligned Nanowires for Longitudinal Intracellular Sampling.” (Under Review)

  • J. Lim, D. Oh, M. Cheng, U. Chintapula, S. Liu, D.E. Reynolds, X. Zhang, X. Xu, J. Ko. “Enhancing Chimeric Antigen Receptor T-Cell Generation via Microfluidic Mechanoporation and Lipid Nanoparticles.” (Under Review)

  • D.E. Reynolds, Y. Roh, D. Oh, P. Vallapureddy, J. Ko. “Temporal and Spatial Omic Technologies for 4D Profiling.” (In Press)

2024

  • Y. Roh*, RT. Morales*, E. Huynh, U. Chintapula, D.E. Reynolds, R.J. Agosto Nieves, D. Oh, A.J. Seiner, J. Lim, C. B. Rodell, J. Ko. “Squeezable Hydrogel Microparticles for Single Extracellular Vesicle Protein Profiling.” Small.

  • L. Huang, J. Yang, J. Zhu, H. Wang, L. Dong, Y. Chen, F. Zhang, Y. Guo, L. Ou, L. Guan, X. Zhu, D. Xu, S. Koosha, J. Xu, Z. Xiao, W. Zhong, E. Burkova, X. Zhang, K. Smith, Y. Fan, J. Ko, C. Liang, M. Herlyn, W. Guo, X. Xu, and S. Liu. “PD-L1 in melanoma and extracellular vesicles promotes local and regional immune suppression through M2-like macrophage polarization.” The American Journal of Pathology.

  • D. Koo, X. Cheng, S. Udani, D. Zhu, J. Li, B. Hall, N. Tsubamoto, J. Ko, K. Cheng, D. Di Carlo. “Increasing Cell Therapeutic Potential by Selecting Cells with High Extracellular Vesicle Secretion.” Nature Communications.

  • Z. Tang, D.E. Reynolds, C. Lv, D. Zhang, J. Ko, Y. Wang. "A Versatile Microchannel Array Device for Portable andParallel Droplet Generatio." Small Science.

  • D.E. Reynolds, J. Lim, Y. Roh, U. Chintapula, RT. Morales, D. Oh, J. Ko. “Droplet and Digital Microfluidics: Ideation to Implementation.” Elsevier.

  • D.E. Reynolds, Y. Sun, X. Wang, P. Vallapureddy, J. Lim, M. Pan, A. Fernadez Del Castillo, J. C. T. Carlson, M. A. Sellmyer, M. Nasrallah, Z. Binder, D. M. O’Rourke, G. Ming, H. Song, J. Ko. “Live Organoid Cyclic Imaging.” Advanced Science.

  • U. Chintapula, D. Oh, C. Perez, S. Davis, J. Ko. “Anti-cancer bioactivity of sweet basil leaf derived extracellular vesicles on pancreatic cancer cells." Journal of Extracellular Biology.

2023

  • L. Ou, S. Liu, H. Wang, Y. Guo, L. Guan, L. Shen, R. Luo, D. Elder, A. Huang, G. Karakousis, J. Miura, T. Mitchell, L. Schuchter, R. Amaravadi, A. Flowers, H. Mou, Y. Fan, W. Guo, J. Ko, Q. Chen, B. Tian, M. Herlyn, X. Xu. “Patient-Derived Melanoma Organoid Models Facilitate the Assessment of Immunotherapies.” eBiomedicine.

  • D.E. Reynolds, M. Pan, J. Yang, G. Galanis, Y. Roh, RT. Morales, S.S. Kumar, SJ. Heo, X. Xu, W. Guo, J. Ko. “Double Digital Assay for Single Extracellular Vesicle and Single Molecule Detection.” Advanced Science.

  • J. Chen, J. Ko, Y. Wang. “Single-cell and spatially resolved omics: Advances and Limitations.” Journal of Pharmaceutical Analysis.

  • D.E. Reynolds, G. Galanis, Y. Wang, J. Ko. “Single Extracellular Vesicle Analysis using Droplet Microfluidics.” Methods Mol Biol, Springer Nature.

  • D.E. Reynolds, P. Vallapureddy, RT. Morales, D. Oh, M. Pan, U. Chintapula, R. L. Linardi, A. M. Gaesser, K. Ortved, J. Ko. “Equine mesenchymal stem cell derived extracellular vesicle immunopathology biomarker discovery.” Journal of Extracellular Biology.

  • Z. Tang, F. Lv, D.E. Reynolds, S. Zhang, S. Zheng, D. Zhang, J. Ko, Y, Chen, Y. Wang. “Highly Parallel, Wash-free, and Ultrasensitive Centrifugal Droplet Digital Protein Detection in Sub-Microliter Blood.” Lab on a Chip.

2022

  • D.E. Reynolds, O. Lewallen, G. Galanis, J. Ko. “A Customizable and Low-Cost Ultraviolet Exposure System for Photolithography.” Micromachines.

  • RT. Morales, J. Ko, “Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles.” ACS Nano.

  • V. Iyer*, Z. Yang*, J. Ko*, R. Weissleder, D. Issadore, “The Future of Microdiagnostics for Clinical Use.” Lab on a Chip.

  • J. Ko, R. Kohler, K. Lucas, E. Halabi, M. Wilkovitsch, J. C. T. Carlson, R. Weissleder, “In Vivo Click Chemistry Enables Multiplexed Intravital Microscopy.” Advanced Science.

  • J. Ko, M. Wilkovitsch, J. Oh, E. Bolli, M. J. Pittet, D. B. Sykes, H. Mikula, R. Weissleder, J. C. T. Carlson, “Spatiotemporal Multiplexed Immunofluorescence Imaging of Living Cells and Tissues with Bioorthogonal Cycling of Fluorescent Probes.” Nature Biotechnology. (Featured in Penn Today, Nature Bioengineering Community, Schmidt Science Fellows, Penn Bioengineering Blog, Penn Engineering Today)

2021

  • B. Trombetta, S. Kandigian, R. Kitchen, K. Grauwet, P. Webb, G. Miller, C. Jennings, S. Jain, S. Miller, Y. Kuo, T. Sweeney, T. Gilboa, M. Norman, D. Simmons, C. Ramirez, M. Bedard, C. Fink, J. Ko, E. De León Peralta, G. Watts, E. Gomez-Rivas, V. Davis, R. Barilla, J. Wang, P. Cunin, S. Bates, C. Morrison-Smith, B. Nicholson, E. Wong, L. El-Mufti, M. Kann, A. Bolling, B. Fortin, H. Ventresca, W. Zhou, S. Pardo, M. Kwock, A. Hazra, L. Cheng, R. Ahmad, J. Toombs, R. Larson, H. Pleskow, N. Luo, C. Samaha, U. Pandya, P. De Silva, S. Zhou, Z. Ganhadeiro, S. Yohannes, R. Gay, J. Slavik, S. Mukerji, P. Jarolim, D. Walt, B. Carlyle, L. Ritterhouse, S. Suliman, “Evaluation of serological lateral flow assays for severe acute respiratory syndrome coronavirus-2.” BMC Infectious Diseases.

  • J. Ko, Y. Wang, K. Sheng, D. Weitz, R. Weissleder, “Sequencing-based protein analysis of single extracellular vesicles (seiSEQ).” ACS Nano.

2020

  • J. Ko, J. Oh, M. Ahmed, J. Carlson, R. Weissleder, “Ultra-Fast Cycling for Multiplexed Cellular Fluorescence Imaging.” Angewandte Chemie. (Featured as a Hot Paper)

  • J. Ko, Y. Wang, J. Carlson, A. Marquard, J. Gungabeesoon, A. Charest, D. Weitz, M. Pittet, R. Weissleder, “Single Extracellular Vesicle Protein Analysis Using Immuno‐Droplet Digital Polymerase Chain Reaction Amplification.” Advanced Biosystems.

  • R. Weissleder, H. Lee, J. Ko, M. Pittet, “COVID-19 diagnostics in context.” Science Translational Medicine.

  • Z. Yang*, M. LaRiviere*, J. Ko*, T. Christensen, S. S. Yee, J. Till, T. Black, A. Adallah, A. Lin, B. Z. Stanger, D. Issadore, and E. L. Carpenter, “A multi-analyte panel consisting of extracellular vesicle miRNAs and mRNAs, cfDNA, and CA19-9 shows utility for diagnosis and staging of pancreatic adenocarcinoma.” Clinical Cancer Research.

  • Y. Wang, T. Cao, J. Ko, Y. Shen, W. Zong, K. Sheng, W. Cao, L. Cai, YL. Zhou, XX. Zhang, C. Zong, R. Weissleder, D. Weitz, “Dissolvable polyacrylamide beads for high-throughput droplet DNA barcoding.” Advanced Science.

  • S. Muraoka, A. M. DeLeo, M. K. Sethi, K. Yukawa, Z. Yang, J. Ko, J. D. Hogan, Z. Ruan, Y. You, Y. K. Wang, M. Medalla, S. Ikezu, W. Xia, S. Gorantla, H. E. Gendelman, D. Issadore, J. Zaia, T. Ikezu, “Proteomic and Biological Profiling of Extracellular Vesicles from Alzheimer’s Disease Human Brain Tissues.” Alzheimer's & Dementia.

2019

  • J. Ko, M. Hemphill, E. Sewell, J. Shallcross, M. Schweizer, D. K. Sandsmark, R. Diaz-Arrastia, J. Kim, D. F. Meaney, D. Issadore, “Multi-dimensional mapping of brain-derived extracellular vesicle microRNA biomarker for traumatic brain injury diagnostics.” J. of Neurotrauma.

2018

  • J. Ko, M. Hemphill, E. Sewell, YJ. Na, D. K. Sandsmark, S. A. Fisher, E. A. Torre, R. Diaz-Arrastia, J. Kim, D. F. Meaney, D. Issadore, “Diagnosis of traumatic brain injury using miRNA signatures in nanomagnetically isolated brain-derived extracellullar vesicles.” Lab on a Chip. (Selected as a Lab on a Chip Widmer Award Winner, MicroTAS, 2017)

  • J. Ko, N. Bhagwat, T. Black, YJ. Na, J. Kim, E. L. Carpenter, B. Z. Stanger, D. Issadore, “miRNA Profiling of Magnetic Nanopore–Isolated Extracellular Vesicles for the Diagnosis of Pancreatic Cancer.” Cancer Research.

  • J. Ko*, S.N. Baldassano*, P. Loh, K. Kording, B. Litt, D. Issadore, “Machine Learning To Detect Signatures of Disease in Liquid Biopsies - A User’s Guide.” Lab on a Chip. (Featured as a journal content highlights)

2017

  • J. Ko, N. Bhagwat, S. S. Yee, N. Ortiz, A. Sahmoud, T. Black, N. M. Aiello, L. McKenzie, M. O’Hara, C. Redlinger, J. Romeo, E. L. Carpenter, B. Z. Stanger, D. Issadore, “Combining Machine Learning and Nanofluidic Technology to Diagnose Pancreatic Cancer Using Exosomes.” ACS Nano.

  • J. Ko, N. Bhagwat, S. S. Yee, C. Redlinger, J. Romeo, M. O’Hara, A. Raj, E. L. Carpenter, B. Z. Stanger, D. Issadore, “A magnetic micropore chip for rapid (< 1 hour) unbiased circulating tumor cell isolation and in-situ RNA analysis.” Lab on a Chip. (Selected as one of Top 10% papers published in Lab on a Chip)

2016

  • J. Ko, M. Hemphill, D. Gabrieli, L. Wu, R. Yelleswarapu, G. Lawrence, W. Pennycooke, A. Singh, D. Meaney, D. Issadore, “Smartphone-based optofluidic exosome diagnostic for concussion.” Scientific Reports.

  • J. Ko*, V.R. Yelleswarapu*, A. Singh, N. Shah, D. Issadore, “Magnetic Nickel iron Electroformed Trap (MagNET): A master / replica fabrication strategy for ultra-high throughput (> 100mL/hr) immunomagnetic sorting.” Lab on a Chip.

  • J. Ko, E. Carpenter, D. Issadore, “Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro- and nano- based devices.” Analyst.

Media

      2020 “Ultra-fast cycling for multiplexed cellular fluorescence imaging” Invited talk at the Biological Research Information Center (BRIC) Webinar, South Korea.

      2019 A Needle in a Haystack – Developing technologies for early detection of cancer, Schmidt Science Fellows.

      2019 “Jina Ko on ultra-high sensitive diagnostics” Wyss Institute at Harvard University, Boston, MA.

      2019 InSPIREd talk, Schmidt Science Fellows, Oxford, UK.

Patents

      2024 J. Ko, H. Lim, Enhancing Cellular Transfection via Microfluidic Mechanoporation and Carriers, 63/711,253.

2023 J. Ko, D. E. Reynolds, M. Pan, Double Digital Assay For Single Extracellular Vesicle, Single Molecule Detection, 63/515,682.

2019 R. Weissleder, J. Carlson, J. Ko, Ultra-fast cycling for multiplexed cellular fluorescence measurements, 29539-0470P03. (License Agreement with Aperture Bio)

      2019 J. Ko, R. Weissleder, Y. Wang, and D. Weitz, Droplet-based single extracellular vesicle sequencing, 29539-0431P01.

      2015 D. Issadore, J. Ko, V. Yelleswarapu, and M. Muluneh, Magnetic separation filters for microfluidic devices, US-2024-0238802-A1. (Licensed by Chip Diagnostics)

      2014 D. Issadore, J. Ko, Apparatus and methods for making vesicles, 20160001290.